目的:探讨ZnSO4对紫外线诱导的大鼠晶状体氧化损伤的保护作用。方法:离体培养大鼠晶状体,随机分为三组:(1)阴性对照组(正常组);(2)阳性对照组(紫外线照射);(3)实验组(紫外线照射+ZnSO4)。观察晶状体的混浊程度,晶状体组织中GSH-Px和SOD的含量,晶状体上皮细胞中HSP70表达的变化。结果:实验组晶状体混浊程度较同时段阳性对照组轻,差异有统计学意义(P<0.05)。紫外线照射后,晶状体上皮细胞中HSP70的表达明显增加,实验组晶状体上皮细胞中HSP70的表达较同时段的阳性对照组显著增加,差异有统计学意义(P<0.05)。阳性对照组中GSH-Px和SOD含量显著低于同时段阴性对照组,差异有统计学意义(P<0.05);实验组中GSH-Px和SOD含量明显高于同时段阳性对照组,差异有统计学意义(P<0.05)。结论:在晶状体中,ZnSO4可诱导HSP70的表达进而对晶状体的氧化损伤起到保护作用。 Objective: To investigate the protective effect of ZnSO4 on oxidative damage induced by UV in rat lens. Methods: Rats were randomly divided into three groups: (1) negative control group (normal group); (2) positive control group (UV irradiation); (3) experimental group (UV irradiation + ZnSO4). Observe the degree of opacity of lens, the content of GSH-Px and SOD in lens tissue and the expression of HSP70 in lens epithelial cells. Results: The degree of lens opacity in the experimental group was lighter than that of the positive control group in the same period (P <0.05). After ultraviolet irradiation, the expression of HSP70 in lens epithelial cells increased obviously. The expression of HSP70 in lens epithelial cells of experimental group increased significantly compared with the positive control group in the same period (P <0.05). The content of GSH-Px and SOD in the positive control group was significantly lower than that in the negative control group at the same time (P <0.05). The contents of GSH-Px and SOD in the experimental group were significantly higher than those in the positive control group Statistical significance (P <0.05). CONCLUSION: ZnSO4 can induce the expression of HSP70 in the lens and thus protect the lens against oxidative damage.