论文部分内容阅读
姜黄素(curcumin)诱导处理的人成骨肉瘤MG-63细胞,在光镜和电镜观察细胞凋亡的基础上,对hnRNP A2/B1在核基质中存在、分布及其与凋亡相关基因产物在MG-63细胞中的共定位关系进行了研究.经姜黄素处理后,细胞出现染色质凝聚、细胞核固缩、凋亡小体等典型的细胞凋亡形态特征;双向凝胶电泳和质谱鉴定结果显示,hnRNP A2/B1存在于MG-63细胞核基质蛋白组分中,在姜黄素处理后细胞核基质蛋白中表达下调.蛋白质印迹杂交结果,证实hnRNP A2/B1在姜黄素处理前后的MG-63细胞核基质蛋白中的存在及其表达下调变化.免疫荧光显微镜观察显示,hnRNP A2/B1定位于MG-63细胞核基质纤维上,经姜黄素处理后出现分布位置与表达水平变化.激光扫描共聚焦显微镜的观察结果显示,hnRNP A2/B1在MG-63细胞凋亡过程中与Bax、Bcl-2、Fas和p53等基因产物具有共定位关系,且其共定位区域发生了变化.研究结果证实了hnRNP A2/B1定位于核基质纤维上,是一种核基质蛋白,在姜黄素诱导人成骨肉瘤MG-63凋亡过程中的表达与分布变化及其与凋亡相关基因的关系显然对MG-63细胞凋亡具有重要影响,这为深入揭示肿瘤细胞凋亡的机制提供了重要科学依据和深入探索的新方向.
Curcumin-induced human osteosarcoma MG-63 cells were observed under light microscope and electron microscope for the presence and distribution of hnRNP A2 / B1 in nuclear matrix and its correlation with apoptosis-related gene products The colocalization of MG-63 cells was studied.After treated with curcumin, typical morphological features of apoptotic cells such as chromatin condensation, nuclear condensation and apoptotic bodies were observed. Two-dimensional gel electrophoresis and mass spectrometry The results showed that hnRNP A2 / B1 existed in the nuclear matrix protein fraction of MG-63 cells and downregulated in the nuclear matrix proteins after curcumin treatment.Western blot hybridization results showed that hnRNP A2 / The expression of hnRNP A2 / B1 was localized in the nuclear matrix fibers of MG-63 cells, and the location and expression level of hnRNP A2 / B1 was changed after curcumin treatment.Laser scanning confocal microscopy The results showed that hnRNP A2 / B1 co-localized with Bax, Bcl-2, Fas and p53 gene products in the process of MG-63 cell apoptosis, and its colocalization region changed. It is confirmed that hnRNP A2 / B1 is located on nuclear matrix fibers and is a nuclear matrix protein. The expression of hnRNP A2 / B1 in the apoptosis of MG-63 cells induced by curcumin and its relationship with apoptosis-related genes It has an important influence on the apoptosis of MG-63 cells, which provides an important scientific basis and a new exploration direction for further revealing the mechanism of tumor cell apoptosis.