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扁平上皮癌相关抗原(Squamous cell carcinomarelated antigen,SCC)由子宫颈扁平上皮癌患者的肝转移组织提取精制而成,分子量为45000的蛋白质,最近制成SCC RIA药盒。基础研究: 1.孵育时间以室温23℃ 24小时最适合。最低检出浓度为1.5ng/ml,最高为150ng/ml。 2.与CEA 0～500ng/ml及与AFP 0～700ng/ml均未发现有交叉反应。 3.批内的变异系数良好,为4.9～11.4％。批间低浓度变化较大,变异系数为17.7％,中高浓度较好,变异系数在10％以下。 4.三种不同浓度血清平均回收率110±16.3
Squamous cell carcinoma associated antigen (Squamous cell carcinomarelated antigen, SCC) from cervical squamous cell carcinoma of liver metastases extracted from patients with a molecular weight of 45,000 protein, recently made SCC RIA kit. Basic research: 1. Incubation time at room temperature 23 ℃ 24 hours the most suitable. The lowest detectable concentration of 1.5ng / ml, up to 150ng / ml. There was no cross-reaction between 0 ~ 500ng / ml CEA and 0 ~ 700ng / ml AFP. 3. The coefficient of variation within the batch is good, 4.9 ~ 11.4%. The variation of low concentration between batches was larger, the coefficient of variation was 17.7%, the middle-high concentration was better, and the coefficient of variation was below 10%. The average recovery of three different concentrations of serum 110 ± 16.3