该研究以探索油菜秸秆厌氧消化产甲烷潜力为目的,利用一组高效纤维素分解产甲烷菌群在CSTR厌氧反应器内分解定量油菜秸秆41 d,通过监测厌氧发酵过程中的甲烷生产效率,以及纤维素酶活性等指标,评价油菜秸秆厌氧发酵产甲烷的能力。结果表明:油菜秸秆在发酵菌群的作用下能够大量产生沼气,50 g干秸秆厌氧发酵后总产气量为13 200 m L,产气效率达到264 m L/g。在发酵过程中,秸秆纤维素被有效分解,纤维素酶活性和半纤维素酶活性分别达0.63、0.81 U/m L。纤维素酶活性与沼气产量具有良好的相关性,相关性系数达到0.95,表明秸秆纤维素类厌氧发酵产沼气体系内,具有良好纤维素酶活性的菌群对甲烷生产具有重要的意义。 In order to explore the methane potential of anaerobic digestion of rapeseed stalks, a group of highly efficient cellulolytic methanogenic bacteria was used to decompose and quantify rape straw in a CSTR anaerobic reactor for 41 days. By monitoring methane production during anaerobic fermentation Efficiency, cellulase activity and other indicators to evaluate the ability of rape straw to produce methane by anaerobic fermentation. The results showed that biogas could be produced in a large amount under the action of fermentation bacteria. The total biomass of 50 g dry straw after anaerobic fermentation was 13 200 m L and the gas production efficiency was 264 m L / g. During the fermentation process, straw cellulose was effectively decomposed, and cellulase activity and hemicellulase activity reached 0.63 and 0.81 U / m L, respectively. The correlation between cellulase activity and biogas production was good, and the correlation coefficient reached 0.95. It indicated that the microbial community with good cellulase activity in the biogas production by straw cellulosic anaerobic fermentation was of great significance to methane production.