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在20±2℃水温条件下,以100 mg/kg的剂量,对半滑舌鳎连续口灌3 d,采用超高效液相色谱仪(UPLC)测试磺胺多辛在半滑舌鳎血浆及各组织中的残留浓度,研究磺胺多辛在半滑舌鳎体内的残留消除规律。结果表明,在停药1d后,血浆中的质量浓度最高,在血浆、肌肉、肝脏、肾脏和皮肤中的质量浓度分别为241.5、50.9、16.66、53.15和97.69μg/g;在停药30 d后,磺胺多辛的质量浓度分别为0.30、0.10、0.05、0.08和0.14μg/g。在血浆和各组织中的残留消除方程分别为C血浆=205.8e-0.21t、C肌肉=56.44e-0.21t、C肝脏=19.94e-0.22t、C肾脏=56.86e-0.23t、C皮肤=117.5e-0.23t。中国农业部NY 5070—2002《无公害食品水产品中渔药残留限量》规定的磺胺药的残留限量低于0.1 mg/kg,若低于此限量值,则半滑舌鳎的血浆、肌肉、肝脏、肾脏和皮肤则分别需要36.3、30.2、24.1、27.6和30.7 d。因肌肉和皮肤是可食性组织,且考虑血浆的影响,最终确定在本实验条件下,磺胺多辛在半滑舌鳎体内的休药期不低于37 d。
Under the conditions of water temperature of 20 ± 2 ℃, 100 mg / kg dose of continuous oral irrigation for 3 days, using ultra performance liquid chromatography (UPLC) to test the residual concentration of sulfadoxine in the smooth muscle and tissues of the smooth tongue sole, To study the elimination of sulfadoxine residues in the body of half smooth tongue. The results showed that after 1 day of drug withdrawal, the highest concentration of plasma was found in plasma, the mass concentrations in plasma, muscle, liver, kidney and skin were 241.5, 50.9, 16.66, 53.15 and 97.69 μg / g, respectively. The mass concentrations of sulfadoxine were 0.30, 0.10, 0.05, 0.08 and 0.14 μg / g, respectively. Residual elimination equations in plasma and each tissue were C plasma = 205.8e-0.21t, C muscle = 56.44e-0.21t, C liver = 19.94e-0.22t, C kidney = 56.86e-0.23t, C skin = 117.5e-0.23t. Chinese Ministry of Agriculture NY 5070-2002 “pollution-free food and aquatic products in the limits of residues of sulfamethoxazole residues” limit of less than 0.1 mg / kg, if less than this limit, the half-smooth tongue lobe plasma, muscle, liver, kidney And skin respectively require 36.3, 30.2, 24.1, 27.6 and 30.7 d. Because of muscle and skin is edible tissue, and taking into account the impact of plasma, and ultimately determine the conditions in this experiment, sulfadoxine in the semi-smooth tongue gall bladder withdrawal period of not less than 37 d.