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目的:通过氟他胺诱导小鼠隐睾动物模型的建立,探讨其睾丸组织的胰岛素样因子3(INSL-3)mRNA表达的变化。方法:妊娠BALB/c小鼠随机分成A、B、C、D、E 5组,每组9只(雄鼠3只,雌鼠6只),合笼交配;分别在雌鼠孕第12~21天持续10 d给予氟他胺灌胃,剂量依次为0、150、300、500、700 mg/kg;分别在出生后第4周和第8周时运用荧光定量PCR技术检测子代小鼠睾丸组织中INSL-3 mRNA的表达情况。结果:A组小鼠无隐睾,B、C组有单侧隐睾,发生率分别10.0%和25.0%;D、E组有双侧隐睾的发生,发生率分别为21.1%和40.0%。随着给药剂量的增大,INSL-3 mRNA的表达逐渐降低;与对照组相比,B、C组表达强度无明显变化(P>0.05);D、E组表达强度明显低于A组(P<0.05)。结论:剂量为150与300 mg/kg的氟他胺能诱导单侧隐睾模型,剂量为500与700 mg/kg的氟他胺能诱导双侧隐睾模型;并且隐睾小鼠睾丸的INSL-3表达随氟他胺浓度升高水平明显下降,可能是隐睾发生的机制之一。
OBJECTIVE: To investigate the alteration of insulin-like factor 3 (INSL-3) mRNA expression in testis induced by flutamide in mouse models of cryptorchidism. Methods: Pregnant BALB / c mice were randomly divided into A, B, C, D and E 5 groups, 9 in each group (3 males and 6 females) Flutamide was administered intragastrically on days 21 and 21 after dosing for 0, 150, 300, 500 and 700 mg / kg, respectively. On the 4th and 8th week after birth, offspring mice INSL-3 mRNA expression in testis. Results: A group of mice without cryptorchidism, B, C group unilateral cryptorchidism, the incidence rates were 10.0% and 25.0%; D, E group had bilateral cryptorchidism, the incidence rates were 21.1% and 40.0% . Compared with the control group, the expression of INSL-3 mRNA had no significant change (P> 0.05), while the expression of INSL-3 mRNA in group D and E was significantly lower than that in group A (P <0.05). CONCLUSION: Flutamide at doses of 150 and 300 mg / kg induces unilateral cryptorchidism and flutamide at doses of 500 and 700 mg / kg induces bilateral cryptorchidism; and INSL -3 expression with the increase in the concentration of flutamide significantly decreased, may be one of the mechanisms of cryptorchidism.