Based on analysis of three alloys with various Re contents, the effects of Re on the interfacial structure characteristic and interfacial dislocations of γ/γ′ in single crystal superalloys were investigated. A high resolution transition electron microscopy (HRTEM) technique was used to detect the structure characteristic of γ/γ′ interface. The interfacial dislocations of γ/γ′ were analyzed with TEM. The results show that as Re content increases, the interfaces of γ/γ′ become orderly, the atomic arrangements at interfaces are more uniform, the number of mismatch dislocations increases and transition areas between γ and γ′ phases become narrow. That Re changes the interfacial structure promotes the formation of dense, regular and homogeneous interfacial dislocation networks in short time during creep at 1 100 ℃ and 140 MPa, which results in strengthened γ/γ′ interface at elevated temperature.