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为了寻求敏感性强、特异性好的检测技术,以用于国内的临床实验,我们应用浅盘凝集试验(TAT)、精子制动试验(SIT)、免疫珠试验(IBT)、酶联免疫吸附试验(ELISA)及间接血凝试验(IHA)5种方法对本所不育门诊病人血清200份和正常生育者血清60份进行了抗精子抗体的测定。结果提示以TAT、SIT、及IBT3种实验结果进行综合评价作为临床诊断的依据对免疫性不育的诊治具有重要的指导意义。
In order to find a sensitive and specific detection technique for clinical trials in China, we used shallow plate agglutination test (TAT), sperm brake test (SIT), immunobead test (IBT), enzyme linked immunosorbent assay (ELISA) and indirect hemagglutination test (IHA) five methods of infertility clinics in our hospital serum 200 and normal fertile serum 60 were tested for anti-sperm antibodies. The results suggest that TAT, SIT, and IBT three kinds of experimental results for comprehensive evaluation as a basis for clinical diagnosis of immune infertility has important guiding significance.