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坛紫菜叶状体经一定剂量的60Co-γ射线辐射再培养一段时间后,出现了少量色素体颜色发生变异的细胞,它们呈点状无规则地镶嵌在野生型细胞中。培养3周后,色素变异细胞分裂并形成了不同颜色的细胞块, 其颜色呈桔红、桔黄、浅黄褐、浅红、紫红、紫褐、黄绿、绿色等。在辐射剂量0-1 100 Gy范围内,叶状体上色素变异细胞块的出现频率随着辐射剂量的增加而增加;但辐射剂量增至1400 Gy时,色素变异细胞块出现的频率反而下降,说明1 100 Gy是合适的辐射剂量。在同一个叶状体的不同部位上色素变异细胞块出现的频率是不同的,从基部到稍部,随着部位的上移而逐渐增加。用酶解法把含色素变异细胞的叶状体细胞单个分离出来并进行离体培养,在它们的再生体中,出现了多种单色的色素突变叶状体,从中分离到tan(红褐色)、bor(深结红色)、ceb(紫红色)、hon(枯草色)、cac(深黄绿色)等不同颜色的纯色突变体。从各单色突变体中分离出来的丝状体,其颜色与各自的叶状体相同。各突变体的丝状体成熟后,放散的壳孢子长成单色的F1叶状体,其颜色与各自最初的母体叶状体相同,说明所得到的上述突变体是稳定的色素突变体。
After a certain dose of 60Co-γ-ray irradiation and re-culturing for a period of time, a small amount of cells with variable color of pigment body appeared, and they were irregularly embedded in the wild-type cells. After 3 weeks of culture, the pigmented mutant cells divide and form cell blocks of different colors. The colors of the pigmented cells are orange, orange, light yellowish brown, light red, purple, purple brown, yellowish green and green. In the range of 0-1 100 Gy, the frequency of appearance of pigmented cell blocks in the fronds increased with the increase of the radiation dose. However, the frequency of appearance of the pigmented variant cells decreased when the radiation dose was increased to 1400 Gy, Description 1 100 Gy is the appropriate radiation dose. In different parts of the same fronds, the appearance of pigmentary aberrant cell lumps is different, ranging from base to slightly, and gradually increasing as the part moves upwards. Enzymatic hydrolysis of the pigment-containing cells of the frond cells individually isolated and cultured in vitro, in their regeneration, there have been a variety of monochromatic pigment mutant fronds, isolated from tan (red-brown) , Bor (deep red), ceb (magenta), hon (hay color), cac (dark yellow-green) and other solid color mutants of different colors. Filaments isolated from monochromatic mutants have the same color as their respective fronds. After the filaments of each mutant matured, the excreted conchospores grew into monochromatic F1 fronds with the same color as the original maternal frond, indicating that the resulting mutant was a stable mutant.