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目的建立通便类保健食品中番泻苷A和番泻苷B含量测定的高效液相色谱方法。方法样品经0.1%碳酸氢钠溶液超声提取后,过滤膜后直接测定,以伊利特C18柱为分析柱;以含有5 mmol/L四庚基溴化铵的乙腈-pH 5.0乙酸盐缓冲液(35∶65,v/v)为流动相;检测波长为340 nm,柱温40℃,流速为1.0 ml/min。结果番泻苷A在13.8μg/ml~413μg/ml范围内与峰面积之间呈良好的线性关系,加标回收率为98.07%,RSD=1.2%;番泻苷B在21.2μg/ml~637μg/ml范围内与峰面积之间呈良好的线性关系,加标回收率为100.09%,RSD=1.4%;且番泻苷A和番泻苷B的重复性、稳定性都较好。结论所建立的番泻苷A和番泻苷B测定HPLC方法,简便、准确,可用于含番泻叶保健食品的质量控制。
Objective To establish a high performance liquid chromatography (HPLC) method for the determination of sennoside A and sennoside B in laxative health food. Methods The samples were ultrasonically extracted with 0.1% sodium bicarbonate solution and the membrane was directly filtered after the membrane was filtered. Elliott C18 column was used as the analytical column. Acetonitrile-pH 5.0 acetate buffer containing 5 mmol / L tetraheptylammonium bromide (35:65, v / v) as mobile phase. The detection wavelength was 340 nm and the column temperature was 40 ℃ with a flow rate of 1.0 ml / min. Results Sennoside A showed a good linear relationship with the peak area in the range of 13.8μg / ml ~ 413μg / ml with the recoveries of 98.07% and RSD of 1.2%, and those of sennoside B at 21.2μg / ml ~ 637μg / ml range and peak area showed a good linear relationship between the recovery of 100.09%, RSD = 1.4%; and sennoside A and sennoside B repeatability and stability are better. Conclusions The established HPLC method for the determination of sennoside A and sennoside B is simple and accurate and can be used for the quality control of senna health food.