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Nitric oxide has played an important role in many physiological and pathological processes as a kind of important gas signal molecules. In this work, a new fluorescent probe LysoNO-Naph for detecting NO in lysosomes based on 1,8-naphthalimide was reported. LysoNO-Naph has sub-groups of o-phenylenediamine as a NO reaction site and 4-(2-aminoethyl)-morpholine as a lysosome-targetable group. This probe exhibited good selectivity and high sensitivity(4.57 mmol/L) toward NO in a wide pH range from4 to 12. Furthermore, LysoNO-Naph can be used for imaging NO in lysosomes in living cells.
Nitric oxide has played an important role in many physiological and pathological processes as a kind of important gas signal molecules. In this work, a new fluorescent probe LysoNO-Naph for detecting NO in lysosomes based on 1,8-naphthalimide was reported. LysoNO- Naph has sub-groups of o-phenylenediamine as a NO reaction site and 4- (2-aminoethyl) -morpholine as a lysosome-targetable group. This probe shows good selectivity and high sensitivity (4.57 mmol / L) toward NO in a wide pH range from 4 to 12. Furthermore, LysoNO-Naph can used for imaging NO in lysosomes in living cells.