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海藻糖是麦红吸浆虫Sitodiplosis mosellana(Gehin)滞育期间储藏能量的主要物质,为弄清其在滞育期的积累机理,本文测定了麦红吸浆虫滞育前后和滞育期糖原磷酸化酶(Gpase)、己糖激酶(HK)、磷酸果糖激酶(PFK)和醛缩酶(ALD)这4种糖代谢酶活力的变化。结果表明:麦红吸浆虫滞育前后这些糖代谢酶活力明显不同,滞育后Gpase活力显著提高,糖酵解有关酶HK,PFK和ALD活力降低;滞育解除后,Gpase活力降低,HK,PFK和ALD活力升高。滞育期间,4种糖代谢酶的活力均与滞育发育有关;同时Gpase和PFK的活力也与环境温度有关,即夏、冬季高于春、秋季;同期不同滞育状态幼虫比较,裸露幼虫HK,PFK和ALD活力总是略高于结茧幼虫,Gpase则相反。滞育当年与第2年同期幼虫4种糖代谢酶的活力无显著差异。
Trehalose is the main energy storage substance during diapause of Sitodiplosis mosellana (Gehin). To clarify its accumulation mechanism during diapause period, trehalose was measured before and after diapause and diastolic glycogen phosphorylase (Gpase), hexokinase (HK), phosphofructokinase (PFK) and aldolase (ALD). The results showed that the activities of these enzymes were significantly different before and after diapair diapause, the activity of Gpase significantly increased after diapause, and the activities of enzymes HK, PFK and ALD in glycolysis decreased. After diapause elimination, the activities of Gpase, HK, PFK And ALD vitality increased. During diapause, the activities of four carbohydrate metabolizing enzymes were all related to diapause development. At the same time, the activities of Gpase and PFK were also correlated with the ambient temperature, that is, the summer and winter were higher than those in spring and autumn. HK, PFK and ALD vitality is always slightly higher than the cocoon larvae, Gpase the opposite. There was no significant difference in the activities of four kinds of carbohydrate metabolizing enzymes in the larvae of diapause and the second year of diapause.