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以荷叶离褶伞菌株为试材,采用L9(34)正交实验设计,在PDA基础培养基上测定了4种无机盐及其正交组合对其菌丝生长的影响,以期为其优质栽培提供理论依据。结果表明:荷叶离褶伞菌丝萌发与生长的最适组合为ZnSO42.0g/L、MgSO44.0g/L、KH2PO44.0g/L、FeSO41.5g/L;高浓度的ZnSO4、MgSO4、KH2PO4以及FeSO4对菌丝生长有抑制作用,其中MgSO4浓度≥10.0g/L时,菌丝停止生长;4种无机盐协同作用对荷叶离褶伞菌丝生长影响的大小依次为FeSO4>MgSO4>KH2PO4>ZnSO4;各组合之间的菌丝生长速度差异极显著,不同组合间有明显的交互作用,最佳组合为2.0g/L ZnSO4+6.0g/L MgSO4+6.0g/L KH2PO4+1.0g/L FeSO4。
In order to study the effect of four inorganic salts and their orthogonal combinations on the mycelial growth of PDA, the orthogonal design of L9 (34) Provide a theoretical basis for cultivation. The results showed that ZnSO42.0g / L, MgSO44.0g / L, KH2PO44.0g / L and FeSO41.5g / L were the most suitable combinations for the germination and growth of A. japonicus. ZnSO4, MgSO4, KH2PO4 And FeSO4 inhibited the mycelial growth. When the concentration of MgSO4 was ≥10.0g / L, the mycelium stopped growing. The effect of four inorganic salts on the growth of the mycelia of Pleurotus citri on in order was FeSO4> MgSO4> KH2PO4 > ZnSO4. The difference of mycelial growth rate was significant between the two combinations, with obvious interaction among different combinations. The best combinations were 2.0g / L ZnSO4 + 6.0g / L MgSO4 + 6.0g / L KH2PO4 + 1.0g / L FeSO4.