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目的 研究短暂缺氧、血清剥夺复氧复注血清 (再灌流 )后有无神经细胞顿抑现象存在及可能的发生机制。方法 将PC12细胞随机分为正常对照组和缺氧组 ,每组根据不同的再灌流时间点又分为 3个亚组 (缺氧组分别为缺氧 15min再灌流 1h组、3h组、6h组 ,正常对照组各亚组的时间点与缺氧组相对应 )。测定短暂缺氧血清剥夺再灌流后不同时间点的三磷酸腺苷含量、线粒体膜电位和细胞活性。结果 与正常对照组相比 ,缺氧血清剥夺 15min再灌流 1h后的三磷酸腺苷含量、线粒体膜电位、细胞活性显著降低 ,差异具有显著性意义 (P <0 .0 5 ) ;缺氧血清剥夺 15min再灌流 6h后基本恢复至正常水平 ,差异无显著性意义 (P >0 .0 5 )。结论 短暂缺氧血清剥夺再灌流后 ,存在低能量状态 ,且可完全恢复 ,表明可能存在神经细胞顿抑现象 ,其原因可能与缺氧血清剥夺再灌流后线粒体呼吸链上的琥珀酸脱氢酶等活性下降及线粒体的膜电位变化等有关。
Objective To study the existence and possible mechanism of transient hypoxia, serum deprivation and reoxygenation serum (reperfusion) after the presence of neuronal stunned phenomenon. Methods PC12 cells were randomly divided into normal control group and hypoxia group. Each group was divided into 3 subgroups according to different reperfusion time points (hypoxia group were reperfused for 1 hour, 3 hours and 6 hours , The normal control group each sub-group of time points and hypoxia group corresponding). The levels of adenosine triphosphate, mitochondrial membrane potential and cell viability at different time points after transient hypoxic serum deprivation and reperfusion were determined. Results Compared with the normal control group, the content of ATP, mitochondrial membrane potential and cell activity decreased significantly after hypoxia serum deprivation for 15 min and reperfusion for 1 h, the difference was significant (P <0.05); hypoxia serum deprived for 15 min Perfused 6h after the return to normal levels, the difference was not significant (P> 0.05). Conclusion After transient hypoxia serum deprivation and reperfusion, there is a low energy state and can be completely recovered, indicating that there may be stunned neuronal cells, the reason may be related to hypoxia serum deprivation and mitochondrial respiratory chain succinate dehydrogenase Such as decreased activity and mitochondrial membrane potential changes and so on.