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以银条为原料,通过乙醇浸提和D101大孔吸附树脂初步纯化,制备得银条α-GOS含量为76.39%,其中水苏糖占86.03%。采用体外厌氧粪样混合培养、荧光原位杂交技术和高效液相色谱法分析银条α-GOS对肠道菌群以及短链脂肪酸(SCFA)生成的影响。结果表明:银条α-GOS能促进双歧杆菌和乳酸菌的增殖,而对拟杆菌和梭状菌的增殖却有一定的抑制作用;银条α-GOS只是改变了肠道内菌群的组成,而对总体菌群的数量基本没有影响;银条α-GOS能显著刺激肠道微生物生成甲酸、乳酸、乙酸、丙酸和丁酸,提高肠道总SCFA的量,尤其是乳酸和乙酸的生成量。综上所述,银条α-GOS具有较好的肠道益生功能。
Silver bar as raw material, ethanol extraction and D101 macroporous adsorption resin was purified, the silver bar α-GOS content was 76.39%, of which stachyose accounted for 86.03%. Anaerobic manure-like mixed culture in vitro, fluorescence in situ hybridization and high performance liquid chromatography were used to analyze the effects of silver bar α-GOS on intestinal microflora and short chain fatty acids (SCFA) production. The results showed that silver bar α-GOS could promote the proliferation of Bifidobacterium and Lactobacillus, but inhibit the growth of Bacteroidetes and Clostridium in a certain extent. Silver bar α-GOS only changed the composition of intestinal flora, While the amount of total flora had no effect on the total bacterial population; silver bar α-GOS significantly stimulated intestinal microbial generation of formic acid, lactic acid, acetic acid, propionic acid and butyric acid, increase the total amount of intestinal SCFA, especially the formation of lactic acid and acetic acid the amount. In summary, silver bullion α-GOS has good intestinal probiotic function.