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目的 :探讨人类乳头状瘤病毒 (HPV)与声带息肉发生的关系。方法 :对 99例声带息肉HE染色标本进行形态学光镜研究 ,同时对其中 1 1例标本行透射和扫描电子显微镜观察 ;99例声带息肉中随机选择 5 0例标本采用聚合酶链反应 (PCR)技术行HPV DNA检测 ,并以 8例正常人喉粘膜作对照。结果 :99例标本中有 5 5例出现凹空样细胞 ,阳性率为 5 5 .6 % ;凹空样细胞出现与患者性别无关 (P >0 .0 5 ) ,与上皮角化伴增生有关 (P<0 .0 1 )。 5 0例行PCR检测标本中HPV感染阳性率为 6 8% ,其中凹空样细胞 2 8例的HPV阳性率为 92 .9% ,正常人喉粘膜HPV阳性率为 1 2 .5 %。电镜观察与光镜观察基本一致 ,但细胞的凹空样表现不典型。结论 :HPV与声带息肉有明显关系 ,其在声带息肉的发生中极可能充当重要角色。
Objective: To investigate the relationship between human papilloma virus (HPV) and vocal cord polyps. Methods: 99 specimens of vocal cord polyp were examined by morphological light microscopy. Eleven specimens were examined by transmission electron microscopy and scanning electron microscopy. Fifty specimens were randomly selected from 99 cases of vocal cord polyps by polymerase chain reaction (PCR) ) Technology line HPV DNA testing, and 8 cases of normal laryngeal mucosa as a control. Results: Fifty-five of 99 specimens showed concave-like cells, with a positive rate of 55.6%. There was no correlation between the number of concave-shaped cells and patient’s gender (P> 0.05), which was associated with hyperkeratosis (P <0. 01). The positive rate of HPV infection in 50 routine PCR samples was 68%. The positive rate of HPV in 28 cases with concave-shaped cells was 92.9%. The positive rate of HPV in normal laryngeal mucosa was 12.5%. Electron microscopy and light microscopy were basically the same, but the concave cell-like cells showed atypical. Conclusion: There is a clear relationship between HPV and vocal cord polyps, which is likely to play an important role in the occurrence of vocal cord polyps.