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目的探讨乳腺癌组织中β微管蛋白Ⅲ(β-tubulin isotypeⅢ,TUBB3)mRNA表达与化疗效果的关系。方法 89例乳腺癌患者,采用DNA液相芯片技术检测手术切除乳腺癌组织中TUBB3 mRNA表达水平,并依据TUBB3mRNA表达水平分为高表达组和中低表达组;术后采用PAC(多西他赛+吡柔比星+异环磷酰胺)或PEC(紫杉醇+表柔比星+环磷酰胺)方案化疗,每21d为1个周期,化疗结束评价疗效;分析TUBB3 mRNA表达与临床病理特征的关系,比较高表达组和中低表达组化疗效果,非条件logistic回归分析影响化疗效果的因素。结果 89例中TUBB3 mRNA高表达49例(55.06%),低表达18例(20.22%),中表达22例(24.72%);TUBB3mRNA表达在年龄,是否绝经以及雌激素受体、孕激素受体、人表皮生长因子受体2是否阳性上差异均无统计学意义(P>0.05),在是否发生淋巴结转移、组织学分级上差异有统计学意义(P<0.05);89例中26例完成3个周期化疗,63例完成4个周期化疗,总有效率为26.97%;高表达组总有效率(14.29%)低于中低表达组(42.50%)(P<0.05);非条件logistic回归分析结果显示,TUBB3 mRNA高表达(OR=1.729,95%CI:1.339~5.528,P=0.049)、组织学低分级(OR=1.618,95%CI:1.201~4.285,P=0.023)、淋巴结发生转移(OR=1.263,95%CI:1.098~2.796,P=0.047)是影响化疗效果的因素。结论乳腺癌化疗效果与乳腺癌组织TUBB3高表达有关。
Objective To investigate the relationship between the expression of β-tubulin isoform Ⅲ (TUBB3) mRNA and the effect of chemotherapy in breast cancer. Methods Eighty-nine patients with breast cancer were examined with DNA liquid-phase microarray. TUBB3 mRNA expression in breast cancer tissues was detected by immunohistochemistry. TUBB3 mRNA expression was divided into high-expression group and low-expression group. + Pirarubicin + ifosfamide) or PEC (paclitaxel + epirubicin + cyclophosphamide) regimen every 21d for one cycle, the end of chemotherapy to evaluate the efficacy; analysis of TUBB3 mRNA expression and clinicopathological characteristics of the relationship , Compared with high expression group and low expression group chemotherapy effect, non-conditional logistic regression analysis of the factors that affect the effect of chemotherapy. Results TUBB3 mRNA was highly expressed in 89 cases (55.06%), 18 cases (20.22%) in low expression, and 22 cases (24.72%) in TUBB3 mRNA expression. The expression of TUBB3 mRNA in patients with age, menopause and estrogen receptor, progesterone receptor (P <0.05). There was no significant difference between the two groups (P> 0.05). There were significant differences in histological grade between lymph node metastasis and lymph node metastasis 3 cycles of chemotherapy, and 63 cases completed 4 cycles of chemotherapy, the total effective rate was 26.97%; the total effective rate of high expression group (14.29%) was lower than that of moderate low expression group (42.50%) (P <0.05); unconditional logistic regression The results showed that TUBB3 mRNA was highly expressed (OR = 1.729, 95% CI: 1.339-5.528, P = 0.049), histological grade (OR = 1.618,95% CI: 1.201-4.285, P = 0.023) Metastasis (OR = 1.263, 95% CI: 1.098-2.756, P = 0.047) was a factor affecting the efficacy of chemotherapy. Conclusion The effect of chemotherapy in breast cancer is related to the high expression of TUBB3 in breast cancer.