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目的对不同产地、不同表型性状的砂仁Amomi Fructus种质资源进行遗传多样性分析。方法采用简单序列重复区间(ISSR)扩增技术,对来自云南、海南、广东、福建等地区的21个砂仁样品进行遗传多样性及亲缘关系分析;同时,对其中16个阳春砂仁的株高、茎粗、叶片数、叶片大小等部分植物学表型性状进行测定,利用这些数据对各居群砂仁进行聚类分析。结果 ISSR分析结果显示,从60条ISSR引物中筛选出11条用于扩增,共检测出54条DNA条带,多态性位点为22个,多态性条带比率(PPB)可达40.74%,Nei’s基因多样性(H)为0.116 1,Shannon’s多态性信息指数(I)为0.184 2;各居群表型性状差异较小。结论砂仁种质资源的遗传多样性较低。
Objective To analyze the genetic diversity of Amomi Fructus germplasm resources of Amomum villosa from different habitats and phenotypic traits. Methods The genetic diversity and genetic relationship of 21 Amomum villosum samples from Yunnan, Hainan, Guangdong, Fujian and other regions were analyzed by Simple Sequence Repeat (ISSR) amplification. Meanwhile, 16 Amomum villosum strains Height, stem diameter, leaf number, leaf size and other botanical phenotypic traits were measured, the use of these data clustering analysis of each population of Amomum villosum. Results The results of ISSR analysis showed that 11 of the 60 ISSR primers were screened for amplification. A total of 54 DNA bands were detected with 22 polymorphic loci. The polymorphic bands (PPB) 40.74%, Nei’s gene diversity (H) was 0.116 1, Shannon’s polymorphism information index (I) was 0.184 2; Conclusion The genetic diversity of Amomum villosum germplasm resources is low.