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目的 通过观察慢性吗啡 (Mor)依赖大鼠血清性激素水平及性腺组织的病理改变 ,探讨依赖动物的中医证型。方法 建立慢性Mor依赖大鼠模型 ,并设立正常组、自然戒断组和中药复方治疗组 ,对慢性Mor依赖和各对照组大鼠的睾丸、附睾组织进行光镜观察 ,运用放免法检测血清FSH ,LH ,T ,PRL水平的变化。结果 发现依赖和戒断组大鼠的睾丸、附睾出现退行性改变 ,血清LH ,T明显降低 ,PRL显著升高 ,温阳益气、活血化瘀复方逆转依赖大鼠的睾丸、附睾退行性改变 ,恢复紊乱的性激素水平。结论 慢性Mor依赖大鼠睾丸轴出现未老先衰的退行性改变属中医“肾阳虚损”范畴
Objective To observe the serum sex hormone levels and the pathological changes of gonadal tissue in chronic morphine (Mor)-dependent rats and explore the animal-dependent TCM syndromes. Methods A rat model of chronic Mor-dependence was established, and the normal group, natural withdrawal group and traditional Chinese medicine compound treatment group were established. The testis and epididymis tissues of rats with chronic Mor dependency and each control group were observed by light microscopy. The serum FSH was measured by radioimmunoassay. Changes in LH, T, and PRL levels. The results showed that degenerative changes in the testis and epididymis were observed in the dependent and withdrawal groups. The serum LH and T levels were significantly lower, and the PRL was significantly increased. The regression of the Wenyang Qi and Huoxue Huayu compound reversing rats’ testis and epididymis was restored. Disordered sex hormone levels. Conclusion The degenerative changes of premature aging in the testis axis of chronic Mor-dependent rats belong to the category of “negative yang deficiency” in traditional Chinese medicine