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本实验测定了紫珠属药用植物批杷叶紫珠、紫珠及其果实、日本紫珠、大叶紫珠、华紫珠、老鸦糊抑制大鼠脏器体外孵育时自动发生脂质过氧化(LPO)50％的药物浓度(IC_(50)),发现除老鸦糊无抑制作用外,其余药物IC_(50)差别较大,以紫珠果实IC_(50)为最小,且IC_(50)与黄酮含量并不完全一致,进一步测定了枇杷叶紫珠、紫珠果实、紫珠对·OH引发的红细胞LPO及溶血抑制的IC_(50)、·OH引发的脑匀浆LPO抑制的IC_(50)、H_O_2与FeSO_4体系生成·OH的清除(或抑制)的IC_(50)发现IC_(50)大小顺序均为紫珠果实>紫珠>枇杷叶紫珠。结果表明,紫珠果实对体外自动发生LPO反应抑制最强,枇杷叶紫珠则对·OH引发的LPO反应抑制最强。
This experiment determined that lipid peroxidation (LPO) occurs when the purple genus Medicinal plants Pterocarya gladiolus, purple bead and its fruit, Japanese purple bead, purple bead, Chinese red bead, and Laoya paste inhibit rat organ in vitro incubation. At 50% drug concentration (IC_(50)), it was found that in addition to the non-inhibitory effect of ergo paste, the other drugs IC_(50) differed significantly, and the IC_(50) of purplish fruit was the smallest, and IC_(50) and flavonoids content Not completely identical, the IC50 and H_O2 inhibition of brain homogenate LPO inhibition induced by IC50 and OH inhibition of erythrocyte LPO and hemolysis inhibition induced by OH in Zizhu, Zizhu fruit, and Zizhu were further determined. IC50 (50) of the scavenging (or suppression) of OH production by the FeSO4 system was found to be in the order of purple bead fruit> purple bead> purple bead. The results showed that the fruit of purple bead had the strongest inhibitory effect on LPO reaction in vitro, while Zizhu purple bead had the strongest inhibition on the LPO reaction induced by OH.