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在硅胶表面通过乙烯基三甲氧基硅烷接枝,以邻苯二甲酸二丁酯(DBP)为模板分子,丙烯酸为功能单体制备了DBP印迹聚合物。采用紫外光谱法(UV)研究单体与模板间的相互作用,扫描电子显微镜表征印迹聚合物的表面形貌,红外光谱分析聚合物的结构特征。用平衡吸附实验方法研究聚合物对DBP的结合性能,用Scatchard法分析了MIP-DBP的吸附解离常数(Kd)和吸附容量(Q)。结果表明,高亲和位点的解离常数Kd1=1.141 mmol/L,最大表观吸附量Qmax1=159.75μmol/g。低亲和位点的解离常数Kd2=3.697 mmol/L,最大表观吸附量Qmax2=299.66μmol/g。制备的印迹聚合物对DBP的结构类似物邻苯二甲酸二甲酯(DMP)和邻苯二甲酸二甲氧乙酯(DMOP)的分离因子(α)分别为2.04和1.57,表现出较好的选择性识别能力。
The DBP imprinted polymer was prepared by grafting vinyltrimethoxysilane on the surface of silica gel, dibutyl phthalate (DBP) as template and acrylic acid as functional monomer. Ultraviolet spectroscopy (UV) was used to study the interaction between monomer and template. The surface morphology of the imprinted polymer was characterized by scanning electron microscopy. The structural characteristics of the polymer were analyzed by infrared spectroscopy. The binding properties of polymer to DBP were studied by equilibrium adsorption experiment. The adsorption-dissociation constant (Kd) and adsorption capacity (M) of MIP-DBP were analyzed by Scatchard method. The results showed that the dissociation constant Kd1 = 1.141 mmol / L and the maximum apparent adsorption capacity Qmax1 = 159.75μmol / g at high affinity sites. Kd2 = 3.697 mmol / L for low affinity sites and Qmax2 = 299.66 μmol / g for maximum apparent adsorption. The separation factors (α) of the prepared imprinted polymers for the structural analogues of DBP, dimethyl phthalate (DMP) and dimethoxyethyl phthalate (DMOP), were 2.04 and 1.57, respectively, showing better Selective recognition ability.