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目的:探讨类叶升麻苷对痴呆小鼠学习记忆的影响。方法:连续灌服类叶升麻苷10 d后,以东莨菪碱建立小鼠记忆获得性障碍模型,采用行为学实验(跳台法)检测动物学习记忆能力,行为学测试结束后,进行小鼠大脑皮层和海马组织中生化指标检测,包括谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、乙酰胆碱酯酶(TChE)以及脑组织蛋白含量的测定。结果:预先给予小鼠类叶升麻苷10 d可以改善东莨菪碱导致的记忆获得性障碍,与模型组比较,类叶升麻苷可显著延长小鼠跳台潜伏期,减少跳台错误次数;类叶升麻苷可使小鼠脑组织中T-SOD,GSH-Px,TChE活性、脑组织蛋白含量升高,MDA含量降低。结论:类叶升麻苷对东莨菪碱致小鼠学习记忆获得性障碍具有显著的改善作用,其作用机制可能与其对抗小鼠体内自由基生成以及改善中枢胆碱能系统功能有关。
Objective: To investigate the effects of acteoside on learning and memory in dementia mice. Methods: After continuous administration of acteoside for 10 days, the model of memory impairment in mice was established by scopolamine. The behavior learning (jumping method) was used to detect the learning and memory ability of animals. After the behavioral test, the cerebral cortex And the detection of biochemical indexes in hippocampus including glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), malondialdehyde (MDA), acetylcholinesterase (TChE) Determination of tissue protein content. Results: Pretreatment of mice with acteoside for 10 d improved scopolamine-induced memory impairment. Compared with the model group, acteoside significantly prolonged the jumping latency and reduced the number of jumping trips; Glycosides could increase T-SOD, GSH-Px, TChE activity, brain tissue protein content and MDA content in mouse brain. CONCLUSION: Acetone can significantly improve the learning and memory impairment of mice induced by scopolamine, and its mechanism may be related to the anti-free radicals generation and the improvement of central cholinergic system in mice.