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本文比较了疫苗株FR—854和FR—836—w的免疫原性,及其对三寸左右草鱼种的有效免疫剂量。疫苗株FR—854和FR—836—w均有较强的免疫原性,其保护力可达70％以上。但试验结果初步证明,FR—854疫苗比FR—836—w疫苗免疫原性更强。经统计分析,FR—854疫苗的免疫保护力可达88.9±12.0(6)％,血清中和抗体效价为160.5±58.9(6);而FR-838-W疫苗分别只有71.3±14.2(6)％、88.3±26.2(6);二者有显著的差异(0.05>P>0.01)。注射免疫三寸左右的草鱼种,FR—854的免疫剂量在3—5×10~(4.5)TCID_(50)/尾左右,FR—836—w在3—5×10~(7.5)TCID_(50)/尾左右,其免疫保护力可达80％左右,同时我们测得ER—854的半数免疫量为10~(5.1)TCID_(50)/ml。
This article compared the immunogenicity of the vaccine strains FR-854 and FR-836-w and their effective immunization dose against three-inch grass carp species. Vaccine strains FR-854 and FR-836-w have strong immunogenicity, the protective power of up to 70%. However, preliminary test results show that the FR-854 vaccine is more immunogenic than the FR-836-w vaccine. The statistical analysis showed that the immunoprotective power of FR-854 vaccine reached 88.9 ± 12.0 (6)% and the serum neutralizing antibody titer was 160.5 ± 58.9 (6), while that of FR-838-W vaccine was only 71.3 ± 14.2 )%, 88.3 ± 26.2 (6); There was significant difference between them (0.05> P> 0.01). The immunization dose of FR-854 was 3-5 × 10 ~ (4.5) TCID 50 / tail and the FR-836-w was 3-5 × 10 ~ (7.5) TCID_ 50) / tail, the immune protection of up to about 80%, at the same time we measured half of the amount of ER-854 immunization for 10 ~ (5.1) TCID_ (50) / ml.