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目的 探讨内质网分子伴侣GRP94在核酸和蛋白水平的表达与人肺癌的关系。方法 采用逆转录聚合酶链反应 (RT PCR)、免疫组化方法和 /或Western杂交 ,检测 5 4例肺癌患者癌组织及癌旁正常肺组织GRP94在核酸和蛋白水平的表达。结果 在肺癌组织及癌旁正常肺组织中均可检测到GRP94的表达 ,但肺癌组织GRP94在核酸和蛋白水平的表达均明显高于癌旁正常肺组织 ,肺癌组织GRP94mRNA相对水平为 3 4 8± 2 0 6 ,GRP94蛋白水平免疫组化染色为 ++ + + + ,Western杂交为 2 5 6 7± 80 6 ;而癌旁正常肺组织GRP94mRNA相对水平为 2 0 1± 1 83,GRP94蛋白水平为 + + +和 10 8 1± 4 2 3,二者存在显著差异 (P <0 0 5 )。而且GRP94水平高低与肺癌分化程度及临床分期呈明显相关性 ,低分化组明显高于中 高分化组 (P <0 0 5 ) ,临床Ⅲ期明显高于临床Ⅰ、Ⅱ期 (P <0 0 5 )。但GRP94的表达与病理类型间未见明显相关性。结论 GRP94的表达与肺癌的发生及发展密切相关。GRP94可能作为一种新的生物标记物以协助判断肺癌的分化程度和临床分期。
Objective To investigate the relationship between the expression of endoplasmic reticulum molecular chaperone GRP94 at the nucleic acid and protein levels and human lung cancer. Methods RT-PCR, immunohistochemistry and / or Western blot were used to detect the expression of GRP94 in 54 lung cancer patients and normal lung tissues. Results The expression of GRP94 was detected in lung cancer tissues and adjacent normal lung tissues. However, the expression of GRP94 in lung cancer tissues was significantly higher than that in adjacent normal lung tissues. The relative level of GRP94 mRNA in lung cancer tissues was 34.8 ± The level of GRP94 protein was ++ + + +, and the level of GRP94 protein was 25 57 ± 80 6. The relative level of GRP94 mRNA in normal lung tissue adjacent to the tumor was 20 1 ± 1 83, GRP94 protein level was + + + And 10 8 1 ± 4 2 3, respectively (P <0.05). Moreover, the level of GRP94 was significantly correlated with the degree of differentiation and clinical stage of lung cancer. The level of GRP94 was significantly higher in the poorly differentiated group than that in the moderately and well differentiated group (P <0.05). The clinical stage Ⅲ was significantly higher than that in the clinical stage Ⅰ and Ⅱ (P <0.05) ). However, there was no significant correlation between the expression of GRP94 and pathological types. Conclusion The expression of GRP94 is closely related to the occurrence and development of lung cancer. GRP94 may serve as a new biomarker to help determine the degree of differentiation and clinical stage of lung cancer.