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目的 探讨热应激蛋白 70 (HSP70 )水平及其抗体滴度在老年人重症中暑发生、发展中的特点及意义。 方法 应用 Western blot法测定血浆中 HSP70水平 ;应用酶联反应技术经倍比稀释测定血浆 HSP70抗体滴度。 结果 重症中暑的老年、非老年组 HSP70水平分别为 42 2 6± 12 49、385 6± 15 92 ,均明显低于健康的老年、非老年对照组的 6 0 91± 196 1、5 991± 90 5 (均为 P<0 .0 1)。重症中暑的老年组 HSP70抗体 1∶ 10以上阳性率为 80 .6 % ,明显高于其他各组 (均为 P <0 .0 1) ,且死亡的 5例老年患者 H SP70抗体滴度均呈高水平状态。 结论 HSP70表达水平下降及 HSP70自身抗体产生可能与老年人重症中暑的发生有关。
Objective To investigate the characteristics and significance of heat shock protein 70 (HSP70) and its antibody titer in the occurrence and development of severe stroke in the elderly. Methods The level of HSP70 in plasma was determined by Western blot. The titer of plasma HSP70 antibody was determined by enzyme-linked immunosorbent assay (ELISA). Results The levels of HSP70 in elderly and non-elderly patients with severe heat stroke were 42 2 6 ± 12 49,385 6 ± 15 92, respectively, which were significantly lower than those in healthy elderly and 6 0 91 ± 196 1,5 991 ± 90 5 (all P <0. 01). Severe heat stroke in elderly group HSP70 antibody 1:10 or more positive rate was 80.6%, significantly higher than the other groups (all P <0.01), and death in 5 elderly patients H SP70 antibody titers were High level status. Conclusion The decreased expression of HSP70 and the production of HSP70 autoantibodies may be related to the occurrence of severe heat stroke in the elderly.