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【目的】盐生植物盐穗木的液泡膜氢离子焦磷酸酶(vacuolar H~+-Pyrophosphatase,HcVP1)作为质子泵可有效地形成H~+梯度,促进细胞质中多余Na~+的排除。将HcVP1基因导入棉花有望培育出耐盐性提高的转基因棉花新品种。【方法】研究对已获得的HcVP1转基因棉花经卡那霉素抗性筛选、PCR和RT-PCR检测分析后,对检测阳性的转基因棉花植株进行200 mmol/L NaCl胁迫处理4 wks,检测转HcVP1基因棉花的耐盐相关生理生化指标。【结果】盐胁迫处理的转基因棉花植株长势良好,叶绿素、脯氨酸、可溶性糖含量均显著高于非转基因棉花组,丙二醛(MDA)含量低于非转基因棉花组,而抗氧化物酶SOD、POD、CAT等的活性则显著高于非转基因棉花组。【结论】盐穗木HcVP1基因转化棉花可以有效地提高棉花的耐盐能力。
【Objective】 vacuolar H ~ + -Pyrophosphatase (HcVP1), a halophyte halophyte, is used as proton pump to effectively form H ~ + gradient and promote the elimination of extra Na ~ + in cytoplasm. The introduction of HcVP1 gene into cotton is expected to breed new varieties of transgenic cotton with enhanced salt tolerance. 【Method】 The obtained HcVP1 transgenic cotton was screened by kanamycin resistance. After PCR and RT-PCR assays, the transgenic cotton plants tested positive for 4 wks under 200 mmol / L NaCl stress. The expression of HcVP1 Physiological and Biochemical Indexes Related to Salt Tolerance of Gene Cotton. 【Result】 The results showed that the transgenic cotton plants treated with salt stress grew well, the contents of chlorophyll, proline and soluble sugar were significantly higher than those of non-transgenic cotton plants, and the content of malondialdehyde (MDA) SOD, POD, CAT and other activities were significantly higher than non-transgenic cotton group. 【Conclusion】 Transforming HcVP1 gene into cotton can effectively improve the salt tolerance of cotton.