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目的:探索自身免疫性疾病患者血清中抗bFGF自身抗体的存在状况。方法:通过对封闭条件、酶标板类型及抗原用量等条件进行优化,建立了检测自身免疫性疾病患者血清中抗bFGF抗体的ELISA检测方法。采用建立的最佳检测条件对395例自身免疫性疾病患者的血清进行了bFGF自身抗体IgG和IgM的检测。结果:在系统性红斑狼疮(SLE)、类风湿性关节炎(RA)、慢性肾炎、皮肌炎(DM)、不明原因发热(FOU)和特发性血小板减少紫癜(IPT)等患者血清中检测到了高滴度的抗bFGF的IgG、IgM自身抗体,各组的平均值和阳性率均明显高于正常对照组(P<0.05)。结论:在自身免疫性疾病患者体内广泛存在着bFGF的自身抗体,对bFGF自身抗体的调查研究将为自身免疫性疾病的机制的探讨奠定基础。
OBJECTIVE: To explore the presence of anti-bFGF autoantibodies in sera of patients with autoimmune diseases. Methods: The ELISA method for the detection of anti-bFGF antibodies in sera of patients with autoimmune diseases was established by optimizing the conditions of blocking, plate type and antigen dosage. The optimal detection conditions were established for the detection of bFGF autoantibodies IgG and IgM in the serum of 395 patients with autoimmune diseases. RESULTS: In patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), chronic nephritis, dermatomyositis (DM), unexplained fever (FOU) and idiopathic thrombocytopenic purpura (IPT) High titers of anti-bFGF IgG and IgM autoantibodies were detected. The mean and positive rates of each group were significantly higher than those of the normal control group (P <0.05). Conclusion: Autoantibodies to bFGF are widely present in patients with autoimmune diseases, and the investigation of bFGF autoantibodies will lay the foundation for the study of the mechanism of autoimmune diseases.