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为探讨ｂｃｒ／ａｂｌ融合基因对维持细胞生存方面的作用，通过脂质体介导的方法将表达ｂｃｒ／ａｂｌ融合区反义ＲＮＡ片段的重组质粒导入Ｋ５６２和ＢＶ１７３细胞系，对细胞的超微结构、细胞大小的改变以及核ＤＮＡ降解等进行观察。结果：表达ｂｃｒ／ａｂｌ反义ＲＮＡ片段的细胞呈典型的细胞凋亡形态，细胞核ＤＮＡ降解。证实ｂｃｒ／ａｂｌ反义ＲＮＡ片段启动人Ｐｈ＋白血病细胞系凋亡过程。提示ｂｃｒ／ａｂｌ融合基因是Ｐｈ＋白血病细胞存活能力增强的重要因素，也为慢性髓系白血病的治疗指出了新的方向
To investigate the role of bcr / abl fusion gene in maintaining cell survival, a recombinant plasmid expressing the bcr / abl fusion region antisense RNA fragment was introduced into K562 and BV173 cell lines by liposome-mediated method. The ultrastructure of cell , Cell size changes and nuclear DNA degradation were observed. Results: The cells expressing bcr / abl antisense RNA showed typical apoptotic morphology and DNA degradation. Confirmed that the bcr / abl antisense RNA fragment initiates apoptosis in human Ph + leukemia cell lines. It is suggested that the bcr / abl fusion gene is an important factor for enhancing the viability of Ph + leukemia cells and also points out a new direction for the treatment of chronic myeloid leukemia