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Objective:To explore a method of semen storage prior to assessment of spermDNA fragmentation. Methods:This study examined a simplified altative of air-drying semen on a microscope slide and reconstituting in seminal plasma prior to assessment of spermDNA fragmentation using the halosperm?G2 kit.Results:It showed that semen could be air-dried and stored ovight at room temperature with no detrimental effect onDNA quality.A significant correlation between results existed for20 semen samples both air-dried and snap-frozen in liquid nitrogen(r=0.982, P=0.000).A mean difference between the results of only -1.98% confirmed the effectiveness of air-drying compared to snap-freezing.Conclusions:Future studies to refine this technique are required on the effect of extrinsic factors such as the choice of reconstituting medium, and stability over an extended time-frame at different temperatures.