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为揭示小麦纹枯病抗源的遗传多样性,发掘优异的抗性种质,利用沟带接种法对前期筛选出的88份抗性种质进行了3年田间抗性鉴定,鉴定出抗或中抗纹枯病的小麦种质32份。利用分布于全基因组的SSR标记对这些抗源进行了遗传多样性分析,59个SSR标记共检测到308个等位变异,每个标记可以检测到2~13个等位基因,平均5.2个;多态性信息含量(PIC)的变异范围为0.12~0.89,平均为0.61,表明材料的遗传丰富度较高。根据聚类分析和主成分(PCA)分析,32份小麦纹枯病抗源按照遗传相似系数可划分为2个组群,国外引进品种和国内改良品种聚为一类,国内农家品种聚为一类,并且与地理分布特征相符。利用与纹枯病抗性QTL紧密连锁的14个SSR标记对32份抗源进行基因型分析,发现与抗性QTL连锁的2BS上的Xwmc154和7DS上的Xbarc126普遍存在,可用于分子标记辅助选择。在武农148、陕983、陕农78、Coker 983、H-Line、Mason和Compair中仅检测到一个已报道的抗病QTL,而在Tyalt中没有检测到已知抗病QTL,这些材料有可能携带新的纹枯病抗性基因/QTL,可以在育种中加以利用。
In order to reveal the genetic diversity of resistance to wheat sheath blight and to explore excellent resistant germplasm, the resistance of 88 resistant germplasms was identified by resistance test Resistance to sheath blight of wheat germplasm 32. A total of 308 alleles were detected in 59 SSR markers with 2 to 13 alleles per marker with an average of 5.2 using SSR markers distributed throughout the genome. The polymorphism information content (PIC) ranged from 0.12 to 0.89 with an average of 0.61, indicating that the genetic richness of the material was high. According to cluster analysis and principal component analysis (PCA), 32 wheat sheath blight resistance sources could be divided into two groups according to the genetic similarity coefficient. The introduced varieties from abroad and the domestic improved varieties clustered into one category, and the domestic farm varieties clustered into one Category, and consistent with the geographical distribution characteristics. Using 14 SSR markers closely linked to sheath blight resistance, 32 antigens were genotyped and found that Xwc154 on 2BS linked to resistant QTLs and Xbarc126 on 7DS were ubiquitous and could be used for molecular marker-assisted selection . Only one reported disease-resistant QTL was detected in Wunong 148, Shaanxi 983, Shannong 78, Coker 983, H-Line, Mason and Compair, but no known QTLs were detected in Tyalt. These materials have May carry new sheath blight resistance genes / QTLs that can be used in breeding.