通过水蒸气蒸馏法提取荆芥挥发油,由单因素实验、正交实验确定工艺条件为:浸泡时间80 min、加水量500 mL、提取时间5 h;影响因素从主到次为提取时间>浸泡时间>加水量。通过稀释法测定了荆芥挥发油的抑菌性能,结果显示荆芥挥发油对金黄色葡萄球菌、大肠杆菌、白色念珠菌的最低抑制浓度(MIC)分别为6.25、25、50 mg/mL。 Volatile oil from Schizonepeta tenuifolia was extracted by steam distillation. The optimum conditions were as follows: soaking time 80 min, adding water 500 mL, extraction time 5 h; influencing factors from main to secondary extraction time> soaking time > Add water. The antibacterial activity of volatile oil of Nepeta was determined by dilution method. The results showed that the minimum inhibitory concentrations (MICs) of volatile oil of Nepeta to Staphylococcus aureus, Escherichia coli and Candida albicans were 6.25, 25 and 50 mg / mL, respectively.