引言 温州蜜柑是栽培较广的无核柑桔种,其无核性是雄性不育所引起,具有重要的经济价值。但是雄性不育性也给一些遗传育种研究带来了障碍,如花粉形态研究以及利用温州蜜柑作为父本的杂交育种研究等。如果能找到一种较为简便的方法,使其育性得到暂时恢复,获得大量花粉,将有助于上述研究工作的开展。 据岩政研究,温州蜜柑雄性败育发生在四分体时期。花粉母细胞减数分裂时出现70.3％的不正常配对现象,也许是雄性败育的原因。15～20℃的温度可促进小孢子的发 INTRODUCTION Wenzhou Satsuma is cultivated a wide range of seedless citrus species, its non-nuclear is caused by male sterility, has important economic value. However, male sterility has also brought obstacles to some genetic breeding research, such as the research on pollen morphology and hybrid breeding using Wenzhou Satsuma as the male parent. If we can find a more convenient way to get its fertility temporarily restored, access to a large number of pollen, will contribute to the above research work. According to the study of the rock administration, the male abortion of mandarin orange in Wenzhou occurs in the quadripartite period. 70.3% abnormal pairing of pollen mother cells during meiosis may be the cause of male abortion. The temperature of 15 ~ 20 ℃ can promote the microspore hair