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目的:在兔肝VX-2移植瘤模型基础上,进行在体肝肿瘤介入性热化疗,观察介入性热增敏与肝组织及肿瘤组织血管渗透性的关系,探讨介入性热化疗的生物学机制。方法:建立可供实验研究的兔Vx-2移植性肝癌模型30只,随机等分为3组(非灌注组、普通灌注组及热灌注组),在X线监视下,经股动脉插管至肝动脉。进行灌注,灌注液为生理盐水(温度为60℃),液量30mL,15min缓慢推注。灌注结束前5min,经导管推注10mg/L伊文思蓝(EvansBlueEB),2mL/kg,非灌注组直接推注EB,灌注EB后置10min,取出肝脏用生理盐水灌注肝动脉冲洗血管中残留的EB。切取靠近肝门部的小块正常肝组织、瘤组织,称重后,放入1mL甲酰胺液中,置于50℃恒温水浴箱60h,提取液用722型光栅分光光度计测出A620nm,从标准曲线上测出相应的EB含量,以反映该组织毛细血管的渗透性。结果:肿瘤组织与肝组织的EB含量在3组中均有差别(P<0.05);正常灌注组与非灌注组肝组织的EB含量、肿瘤组织的EB含量无明显差别(P>0.05);热灌注组与非灌注组、正常灌注组肝组织的EB含量、肿瘤组织的EB含量有明显差别(P<0.05)。结论:介入热疗可以增加肝组织及肿瘤组织的血管渗透性。
OBJECTIVE: To study the relationship between interventional hyperthermia and vascular permeability in liver and tumor tissues based on the rabbit liver VX-2 xenograft model and to investigate the biology of interventional thermochemotherapy mechanism. Methods: Thirty rabbits with experimental Vx-2 transplanted hepatocellular carcinoma were established and randomly divided into three groups (non-perfusion group, normal perfusion group and heat perfusion group). Under X-ray monitoring, To the hepatic artery. Perfusion, perfusion fluid for the saline (temperature is 60 ℃), liquid volume 30mL, 15min bolus injection. Five minutes before the end of perfusion, Evans Blue E 10 mg / L Evans Blue E (2 mg / L) was injected intraperitoneally. The EB was injected directly into the non-perfusion group and then left for 10 minutes. The residual hepatic artery perfusion EB. Cut small pieces of normal liver tissue and tumor tissue close to the hilar department and put it in 1mL formamide solution and put in a constant temperature water bath at 50 ℃ for 60h. The extract was measured with a 722 grating spectrophotometer to obtain A620nm. From The corresponding EB content was measured on the standard curve to reflect the capillary permeability of the tissue. Results: The content of EB in tumor tissue and liver tissue were different in three groups (P <0.05). There was no significant difference in the content of EB and the content of EB in tumor tissue between normal group and non - perfusion group (P> 0.05). The content of EB in liver tissue and the content of EB in tumor tissue in normal perfusion group and non-perfusion group were significantly different (P <0.05). Conclusion: Interventional hyperthermia can increase the vascular permeability of liver and tumor tissues.