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目的探讨过继转移日本血吸虫感染鼠DC对IL-13细胞因子调控抑制过敏性哮喘黏液分泌的作用机制。方法采用MACS磁珠分选方法从日本血吸虫(SJ)感染小鼠及健康对照小鼠脾脏中提取DC,分别过继转移给BALB/c小鼠,并用OVA诱发哮喘。4周后处死小鼠,提取肺组织总RNA并制作病理切片,采取qRT-PCR和PAS染色方法等检测IL-13mRNA以及蛋白水平。建立DC与CD4+T细胞共培养体系,检测IL-13的表达情况。结果肺组织病理学检查显示,与OVA单纯哮喘组比较,过继转移SJ感染DC组(SJ+OVA)过敏性哮喘被抑制。qRT-PCR显示,过继转移SJ感染DC组IL-13mRNA表达受到抑制。PAS染色显示SJ感染DC组黏液分泌减少,免疫组化检查该组IL-13的表达量降低。DC与CD4+T细胞共培养结果显示SEA处理DC能抑制CD4+T细胞IL-13的表达。结论过继转移日本血吸虫感染鼠DC通过下调CD4+T细胞中IL-13细胞因子的释放而抑制过敏性哮喘的黏液分泌。
Objective To investigate the mechanism of IL-13 cytokines in inhibiting mucosal secretion of allergic asthma induced by adoptive transfer of Schistosoma japonicum in mice. Methods DCs were isolated from the spleens of mice infected with Schistosoma japonicum (SJ) and healthy control mice by MACS. The DCs were adoptively transferred to BALB / c mice and asthma was induced by OVA. After 4 weeks, the mice were sacrificed and the total RNA was extracted from the lung tissue to make pathological sections. The levels of IL-13 mRNA and protein were detected by qRT-PCR and PAS staining. Establishment of DC and CD4 + T cell co-culture system, detection of IL-13 expression. Results Lung histopathological examination showed that allergic asthma was inhibited in adoptive transfer SJ-infected DCs (SJ + OVA) compared with OVA-only asthma group. qRT-PCR showed that the expression of IL-13mRNA was inhibited in adoptive transfer SJ-infected DCs. PAS staining showed that mucosal secretion decreased in SJ-infected DCs and IL-13 expression decreased in immunohistochemistry. DC co-cultured with CD4 + T cells showed that DCs treated with SEA could inhibit the expression of IL-13 in CD4 + T cells. CONCLUSION: DCs infected with Schistosoma japonicum can inhibit the mucus secretion of allergic asthma by down-regulating the release of IL-13 cytokines in CD4 + T cells.