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目的研究硫酸乙酰肝素对糖尿病大鼠创面愈合的影响。方法 36只雄性糖尿病大鼠随机数字法分为两组:实验组(n=18)采用皮下注射0.5ml硫酸乙酰肝素(0.3mg/ml),对照组(n=18)则皮下注射0.5ml生理盐水。两组大鼠麻醉成功后在其背部制作一直径为20 mm的圆形伤口,观察创面愈合情况,并通过苏木精-伊红和免疫组化染色研究两组大鼠伤口肉芽组织厚度和炎症反应情况。结果术后第4、6、8、10、12和14天时实验组伤口愈合率明显快于对照组(P<0.05)。实验组术后3天中性粒细胞相对于对照组较少,肉芽组织无明显水肿;术后7天时中性粒细胞较前减少,伴有局部毛细血管的增生;术后14天时新生肉芽组织基本修复完成,而对照组伤口仍伴有轻度炎症反应。免疫组化结果提示术后第14天实验组伤口肿瘤坏死因子-α阳性面积较对照组明显降低(561.4±18.7 vs 685.0±31.6,P=0.004),血管内皮生长因子阳性面积较对照组明显升高(688.1±19.3 vs 584.2±14.6,P=0.002)。结论硫酸乙酰肝素可能通过增加血管新生和抑制伤口炎症反应,从而促进创面的愈合。
Objective To study the effect of heparan sulfate on wound healing in diabetic rats. Methods 36 male diabetic rats were randomly divided into two groups: experimental group (n = 18), subcutaneous injection of 0.5 ml heparan sulfate (0.3 mg / ml) and control group (n = 18) brine. After successful anesthesia in the two groups, a circular wound with a diameter of 20 mm was made on the back of the wound. The wound healing was observed. The granulation tissue thickness and inflammation of wounds in both groups were studied by hematoxylin-eosin and immunohistochemistry Reaction situation. Results The wound healing rate of the experimental group was significantly faster than that of the control group on the 4th, 6th, 8th, 10th, 12th and 14th days after operation (P <0.05). The number of neutrophils in the experimental group was less than that in the control group on the 3rd day after operation, and there was no obvious edema in the granulation tissue. The number of neutrophils decreased on the 7th day after operation, accompanied by local capillaries hyperplasia; on the 14th day after operation, The basic repair is complete, while the control group wounds are still associated with mild inflammatory reactions. The results of immunohistochemistry showed that the positive area of tumor necrosis factor-α in the experimental group was significantly lower than that in the control group (561.4 ± 18.7 vs 685.0 ± 31.6, P = 0.004) on the 14th day after operation, and the positive area of VEGF was significantly higher than that of the control group High (688.1 ± 19.3 vs 584.2 ± 14.6, P = 0.002). Conclusion Heparan sulfate may promote wound healing by increasing angiogenesis and inhibiting wound inflammation.