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目的分析江西省2005年-2009年季节性H1N1流感病毒M2以及NA基因的特点,掌握其耐药情况,为流感防控提供参考。方法从江西省流感监测网中随机选择26株季节性A(H1N1)流感病毒,经核酸提取和one-step RTPCR扩增M以及NA基因片段,双向序列测定,采用DNAStar 5.0和Mage 4.0序列分析软件分析M2以及NA基因特征以及耐药性位点。结果除2005年分离的3株病毒和2009年分离的2株病毒的M2基因重要位点未发生变异外,其他21株病毒均发生了S31N的氨基酸替换;2009年的5株分离株均发生H274Y突变外,其他21株病毒NA蛋白催化活性位点和辅助位点均未发生氨基酸替换。结论 2005年分离株均对奥司他韦敏感,部分毒株对金刚烷胺类药物耐药;2006年-2008年的分离株对金刚烷胺类药物耐药,但对奥司他韦敏感;2009年的分离株均对奥司他韦耐药,部分毒株对金刚烷胺类药物也耐药,应加强流感病毒耐药性监测。
Objective To analyze the characteristics of M2 and NA genes of seasonal H1N1 influenza virus from 2005 to 2009 in Jiangxi Province and to master their drug resistance so as to provide a reference for prevention and control of influenza. Methods Sixteen influenza A (H1N1) viruses were randomly selected from the influenza surveillance network of Jiangxi Province. The nucleic acids were extracted and one-step RTPCR was used to amplify the M and NA gene fragments. The DNA sequence was analyzed by DNAStar 5.0 and Mage 4.0 sequence analysis software Analysis of M2 and NA gene traits and drug resistance loci. Results The amino acid substitutions of S31N were found in all the 21 other viruses except for the three major M2 isolates isolated in 2005 and the two viruses isolated in 2009. H274Y occurred in all five isolates in 2009 Mutations, the other 21 strains of virus NA protein catalytic site and auxiliary sites did not occur amino acid replacement. Conclusion All the isolates were sensitive to oseltamivir in 2005 and some strains were resistant to amantadine. The isolates were resistant to amantadine but sensitive to oseltamivir in 2006-2008. Isolates from 2009 were resistant to oseltamivir, and some strains were also resistant to amantadine. Monitoring of influenza virus drug resistance should be strengthened.