本文拟建立一种分离滤泡相关上皮 (FAE )的稳定方法 ,并对微皱褶细胞 (M细胞 )的可诱导性作初步探讨。将BALB/c小鼠随机分为 2组 ,每组 11或 2 2只 ,以小鼠灌胃免疫为模型 ,采用Percoll非连续密度梯度离心法提取小鼠小肠Payer结中的FAE ,经荧光标记的凝集素 (UEA1 FITC )染色后用流式细胞仪检测 ,SAS统计软件分析。结果表明伤寒沙门菌Ty2 1a免疫组与PBS对照组之间 ,有极显著性差异 (P <0 0 1)。说明伤寒沙门菌Ty2 1a可诱导微皱褶细胞 (M细胞 )数量的增加 This article intends to establish a method for the isolation of follicular-associated epithelium (FAE) and to investigate the inducibility of micro-wrinkled cells (M-cells). BALB / c mice were randomly divided into 2 groups (11 or 22 mice in each group). The gavage mice immunization was used as a model. Percoll non-continuous density gradient centrifugation was used to extract the FAE from the small intestine. Lectin (UEA1 FITC) staining using flow cytometry, SAS statistical software analysis. The results showed that there was a significant difference (P <0.01) between Salmonella typhi Ty2 1a immunized group and PBS control group. It indicates that Typhi 1a of Typhoid fever Typhimurium can induce the increase of the number of M folds