论文部分内容阅读
目的探讨高尔基蛋白73(GP73)、热休克蛋白27(HSP27)联合甲胎蛋白(AFP)检测在乙型肝炎相关性早期肝细胞癌诊断中的价值。方法选择50例HBV相关早期肝细胞癌患者、50例乙肝肝硬化患者和30名健康人,采用酶联免疫吸附试验(ELISA)检测GP73、HSP27水平,电化学发光法检测AFP含量。实时荧光定量PCR方法测定肝癌患者的HBV-DNA水平。结果早期肝癌组患者血清GP73、HSP27、AFP水平分别为(266.37±98.41)ng/ml、(847.18±211.72)pg/ml、(368.56±65.32)ng/ml,明显高于肝硬化组[(60.23±32.30)ng/ml、(338.46±114.3)pg/ml、(26.22±8.58)ng/ml]、以及对照组[(23.32±11.08)ng/ml、(73.39±38.54)pg/ml、(7.15±3.32)ng/ml],差异有统计学意义(均P<0.01);早期肝癌组患者血清AFP、GP73、HSP27单项检测阳性率分别为75%、82%、74%,及AFP+GP73+HSP27联合检测的阳性率为86%,均明显高于肝硬化组(分别为48%、37.5%、18%、32%)及健康对照组(分别为3%、6%、0、0)(均P<0.01),其中AFP+GP73+HSP27联合检测阳性率最高(P<0.01);AFP、GP73及HSP27与HBV-DNA的相关性为0.076、0.119、0.176(P>0.05)。结论 GP73、HSP27与AFP联合检测可作为诊断肝细胞癌的重要指标,为早期发现及治疗提供依据,它们与HBVDNA情况无关。
Objective To investigate the diagnostic value of GP73, HSP27 and AFP in the diagnosis of hepatocellular carcinoma (HCC) associated with hepatitis B (HBV). Methods Fifty patients with HBV-related early hepatocellular carcinoma, 50 patients with hepatitis B cirrhosis and 30 healthy individuals were enrolled in the study. The levels of GP73 and HSP27 were detected by enzyme-linked immunosorbent assay (ELISA) and AFP content by electrochemiluminescence. Real-time fluorescent quantitative PCR method for the determination of HBV-DNA levels in patients with liver cancer. Results The serum levels of GP73, HSP27 and AFP in patients with early hepatocellular carcinoma were (266.37 ± 98.41) ng / ml, (847.18 ± 211.72) pg / ml and 368.56 ± 65.32 ng / ml, (32.32 ± 11.08) ng / ml, (73.39 ± 38.54) pg / ml, (7.15 ± 0.32) ng / ml, ± 3.32) ng / ml], the difference was statistically significant (all P <0.01). The positive rates of serum AFP, GP73 and HSP27 were 75%, 82%, 74% The positive rate of HSP27 was 86%, which were significantly higher than those in cirrhosis (48%, 37.5%, 18%, 32%, respectively) and healthy controls (3%, 6%, 0% (P <0.01). The positive rate of AFP + GP73 + HSP27 was the highest (P <0.01). The correlation between AFP, GP73 and HSP27 and HBV-DNA was 0.076,0.119,0.176 (P> 0.05). Conclusion Combined detection of GP73, HSP27 and AFP can be used as an important index in the diagnosis of hepatocellular carcinoma and provide a basis for early detection and treatment, which has nothing to do with the situation of HBVDNA.