Driven equilibrium single pulse observation of T1(DESPOT1)is a rapid spin-lattice relaxation constant(T1)mapping technique in magnetic resonance imaging(MRI).However,DESPOT1 is very sensitive to flip angle(FA)inhomogeneity,resulting in T1 inaccuracy.Here,a five-point DESPOTl method is proposed to reduce the sensitivity to FA inhomogeneity through FA measurement and calibra-tion.Phantom and in vivo experiments are performed to validate the technique.As a result.a rapid and accurate T1 mapping is acquired by using the proposed five-point DESPOT1 method.