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目的:探讨葛根素对大鼠心肌缺血再灌注损伤的保护机制。方法:24只雌性Sprague-Dawley(SD)大鼠随机分为假手术组、缺血再灌注组和葛根素干预组,RT-PCR和Western blotting法检测心肌中TLR4 mRNA及TLR4蛋白的表达,同时四唑氮蓝染色测定心肌梗死范围。结果:大鼠心肌缺血再灌注损伤模型造模成功;葛根素干预组的心肌梗死范围明显小于缺血再灌注组(P<0.01),且该组大鼠TLR4mRNA及蛋白的表达也显著低于缺血再灌注组(P<0.01)。结论:葛根素干预可减轻大鼠心肌缺血再灌注的损伤,其机制可能与抑制TLR4的表达相关。
Objective: To investigate the protective effect of puerarin on myocardial ischemia-reperfusion injury in rats. Methods: Twenty-four female Sprague-Dawley (SD) rats were randomly divided into sham operation group, ischemia reperfusion group and puerarin intervention group. The expression of TLR4 mRNA and TLR4 protein in myocardium were detected by RT-PCR and Western blotting, Tetrazole nitrogen blue staining for the determination of infarct size. Results: Myocardial ischemia-reperfusion injury model was successfully established in rats. The extent of myocardial infarction in puerarin-treated group was significantly less than that in ischemia-reperfusion group (P <0.01), and the expression of TLR4 mRNA and protein was also significantly lower in this group Ischemia-reperfusion group (P <0.01). Conclusion: Puerarin can attenuate myocardial ischemia-reperfusion injury in rats. The mechanism may be related to the inhibition of TLR4 expression.