论文部分内容阅读
以苎麻（ＢｏｅｈｍｅｒｉａｎｉｖｅａＬ．）的试管苗为试材，由茎段诱导的愈伤组织分离原生质体，用ＭＴ＋０．５ｍｇ·Ｌ－１ＫＴ＋０．５ｍｇ·Ｌ－１ＧＡ３＋０．０２ｍｇ·Ｌ－１ＮＡＡ＋５０ｍｇ·Ｌ－１水解乳蛋白＋Ｋ８ｐ＋６ｍｇ·Ｌ－１蔗糖的基本培养基以低熔点琼脂糖（ＬＭＴ）软包埋方式培养，５～６天后原生质体开始分裂，２０～２５天形成细胞团，随后产生愈伤组织，５０～６５天能再生成完整植株。对影响苎麻原生质体分离、培养及再生的主要因子进行比较研究的结果表明：用６％的纤维素酶分离苎麻的原生质体效果较好；蔗糖和葡萄糖是比较适宜的碳源；低熔点琼脂糖（ＬＭＴ）软包埋培养方式，其原生质体分裂频率高、再生成株所需的时间短。
Protoplasts were isolated from stems of Boehmerianivea L. by using stem explants of Boehmerianivea L., and then hydrolyzed by MT + 0.5mg · L-1KT + 0.5mg · L-1GA3 + 0.02mg · L-1NAA + 50mg · L-1 The basic medium of milk protein + K8p + 6mg · L-1 sucrose was cultured by soft embedding with low melting point agarose (LMT). After 5-6 days, the protoplasts began to divide and formed cell masses 20-25 days, followed by callus formation. 50 ~ 65 days to regenerate into complete plants. The results showed that protoplast isolation of ramie with 6% cellulase was better; sucrose and glucose were more suitable carbon sources; low melting point agarose (LMT) soft-embedded culture mode, the protoplast division frequency is high, regeneration of the plant required time is short.