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共轭聚合物(CPs)具有强的光捕获能力和独特的荧光信号放大效应,已经被广泛地用于生物大分子的检测以及细胞及动物水平上的荧光成像.在过去的十几年里,为了满足多色荧光检测及成像的需要,科研工作者设计并发展了一系列基于共轭聚合物的荧光共振能量转移(CPs-FRET)的多色荧光体系.这类多色荧光体系具有荧光强度高、光稳定性好以及细胞毒性低的优点.利用这类体系在单波长的激发光激发下即可实现目标分子的多色荧光检测及多色荧光成像.本文阐述了CPs的FRET机制以及基于CPs-FRET的多色荧光体系的构建方法,同时介绍了近期这类体系在生化检测和细胞成像领域中的研究进展以及目前所存在的问题和未来的发展展望.
Conjugated polymers (CPs) have strong light trapping ability and unique fluorescence signal amplification effect and have been widely used in the detection of biological macromolecules and fluorescence imaging at the cellular and animal level.In the past ten years, In order to meet the needs of multi-color fluorescence detection and imaging, researchers have designed and developed a series of multicolor fluorescent systems based on conjugated resonance fluorescence energy transfer (CPs-FRET) of polymers.This kind of multicolor fluorescent system has fluorescence intensity High light stability and low cytotoxicity.Using this kind of system can achieve the multi-color fluorescence detection and multi-color fluorescence imaging of the target molecule under the excitation of single wavelength excitation light.This paper describes the FRET mechanism of CPs, CPs-FRET multi-color fluorescence system construction methods, and also introduced the recent biosensing of such systems in the field of biomedical detection and cell imaging research and the current problems and future development prospects.