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目的研究采用HPLC法测定复方酸枣仁软胶囊中阿魏酸和五味子甲素的方法。方法阿魏酸定量测定的色谱条件:色谱柱为迪马公司C18柱(200 mm×4.6 mm,5μm),流动相为甲醇-1%醋酸水溶液(35∶65),检测波长为320 nm,体积流量为1 mL/min,柱温为室温,进样量为10μL;理论塔板数按阿魏酸峰计不低于3 000;五味子甲素定量的色谱条件:色谱柱为迪马公司C18柱(200 mm×4.6 mm,5μm);流动相为甲醇-水(77∶33),检测波长为254 nm,体积流量为1 mL/min,柱温为室温,进样量为10μL。结果阿魏酸的线性范围9.8～49.0μg/mL(r=0.999 6);加样回收率试验RSD为1.98%;五味子甲素的线性范围8.8～44.0μg/mL(r=0.999 9);加样回收率试验RSD为0.98%。结论本方法准确、简便、可靠,可作为复方酸枣仁软胶囊质量控制的方法。
Objective To study the method for the determination of ferulic acid and schisandrin A in Fufang Suanzaoren soft capsule by HPLC. Methods The chromatographic conditions for the quantitative determination of ferulic acid were as follows: the column was Dima C18 column (200 mm × 4.6 mm, 5 μm), the mobile phase was methanol-1% acetic acid aqueous solution (35:65), the detection wavelength was 320 nm, The flow rate of 1 mL / min, the column temperature was room temperature, the injection volume was 10μL; the theoretical plate number of ferulic acid peak is not less than 3 000; Schisandra A quantitative chromatographic conditions: column Dima C18 column (200 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (77:33). The detection wavelength was 254 nm, the volume flow rate was 1 mL / min, the column temperature was room temperature and the injection volume was 10 μL. Results The linear range of ferulic acid was 9.8-49.0 μg / mL (r = 0.999 6). The RSD of recovery was 1.98%. The linear range of deoxyschizandrin was 8.8-44.0 μg / mL (r = 0.999 9) The sample recovery test RSD was 0.98%. Conclusion This method is accurate, simple and reliable, and can be used as a quality control method for compound Suanzaoren soft capsules.