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目的:为提高抗核抗体(ANA)阳性率。方法:观察两种抗原底物(Hep-2细胞涂片即人喉癌上皮细胞和鼠肝细胞冰冻切片)在各种风湿性疾病患者中的阳性率。结果:在系统性红斑狼疮(SLE)患者中,以Hep-2细胞为底物时ANA阳性率是98.8%,而以鼠肝切片为底物时阳性率是91.4%,两者之间相比无统计学意义;在干燥综合征(SS)、类风湿关节炎(RA)、硬皮病(SSc)患者中,以Hep-2细胞和鼠肝切片为底物ANA阳性率分别为:81.2%和65.0%,44.6%和19.4%、60.7%和32.3%,两种底物的ANA检测率差异有显著性。结论:ANA检测优先考虑以Hep-2细胞为抗原底物。
Objective: To improve the anti-nuclear antibody (ANA) positive rate. Methods: The positive rates of two antigenic substrates (Hep-2 cell smears, human laryngeal carcinoma epithelial cells and mouse hepatocytes frozen sections) were observed in patients with various rheumatic diseases. Results: The positive rate of ANA in Hep-2 cells was 98.8% in systemic lupus erythematosus (SLE) patients and 91.4% in rat liver slices as substrate The positive rates of ANA with Hep-2 cells and murine liver slices as substrates in patients with Sjogren’s syndrome (SS), rheumatoid arthritis (RA) and scleroderma (SSc) were 81.2% And 65.0%, 44.6% and 19.4%, 60.7% and 32.3% respectively. There was a significant difference in ANA detection rate between the two substrates. Conclusion: ANA test gives priority to Hep-2 cells as antigen substrate.