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目的:研究白桦酸(BA)对人胰腺癌细胞(BxPC-3)增殖、迁移、细胞周期和凋亡的影响。方法:磺酰罗丹明B(SRB)方法测定BA对BxPC-3细胞的增殖抑制率,通过显微镜观察BxPC-3形态变化,用划痕法检测BxPC-3的迁移能力,流式细胞术检测BA对BxPC-3细胞周期的影响,Hoechst33342-PI双染色法观察细胞凋亡,Western Blot印迹检测Bcl-2,Bax的表达。结果:BA在体外明显抑制BxPC-3细胞的增殖,IC50为16.54 mg.L-1,BA在5 mg.L-1时即能抑制细胞的迁移;流式细胞术分析表明,BA可将BxPC-3细胞阻滞于G0期;Hoechst33342-PI双染显示,当BA质量浓度提高到20 mg.L-1以上,可见明显的凋亡细胞和死亡细胞;Western blot的结果说明,随着BA浓度的递增,Bax表达逐渐增加,Bcl-2表达逐渐减少,Bcl-2/Bax下降,并呈剂量依赖性。结论:BA具有明显抑制BxPC-3细胞增殖和迁移,诱导细胞凋亡的作用,有可能成为一个有效的治疗人胰腺癌药物。
AIM: To investigate the effects of betulinic acid (BA) on the proliferation, migration, cell cycle and apoptosis of human pancreatic cancer cells (BxPC-3). Methods: The proliferation inhibition rate of BxPC-3 cells was determined by the method of sulforhodamine B (SRB). The morphological changes of BxPC-3 cells were observed by microscope. The migration of BxPC-3 was detected by scratch assay. On the cell cycle of BxPC-3 cells. Hoechst33342-PI double staining was used to observe the apoptosis. Western Blot was used to detect the expression of Bcl-2 and Bax. Results: BA inhibited the proliferation of BxPC-3 cells in vitro with an IC50 of 16.54 mg.L-1. The BA inhibited the migration of cells at a dose of 5 mg.L-1. Flow cytometry analysis showed that Bax -3 cells arrested in G0 phase; Hoechst33342-PI double staining showed that when BA mass concentration increased to above 20 mg.L-1, obvious apoptotic cells and dead cells were observed; Western blot results showed that with BA concentration , The expression of Bax gradually increased, the expression of Bcl-2 decreased gradually, Bcl-2 / Bax decreased, and in a dose-dependent manner. CONCLUSION: BA can significantly inhibit the proliferation and migration of BxPC-3 cells and induce the apoptosis of BxPC-3 cells. It may be an effective drug for the treatment of human pancreatic cancer.