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从水稻(Oryza sativa L.)、烟草(Nicotiana tabacum L.)和黑松(Pinus thunbergii Parl.)等植物的22对叶绿体SSR引物中筛选出5对能用于柑橘叶绿体SSR分析的引物,应用这5对引物对9个组合的柑橘体细胞杂种的叶绿体遗传进行了分析。结果表明:这些组合再生的杂种中叶绿体都呈现随机分离,该现象与以前报道的RFLP分析结果一致,而且其可靠性已被CAPS分析所证实。表明柑橘叶绿体SSR同RFLP及CAPS一样可靠,并且更简单高效、易于操作,特别适合对柑橘等植物体细胞杂种进行早期胞质遗传组成分析。
From 22 pairs of chloroplast SSR primers from plants such as Oryza sativa L., Nicotiana tabacum L. and Pinus thunbergii Parl., Five pairs of primers for SSR analysis of chloroplast in citrus were screened. 5 pairs of primer pairs of 9 combinations of citrus somatic hybrid chloroplast genetic analysis. The results showed that the chloroplasts were randomly separated in the hybrids regenerated from these combinations. This phenomenon was consistent with the previously reported results of RFLP analysis, and its reliability has been confirmed by CAPS analysis. It indicated that the chloroplast SSR of citrus chloroplast was as reliable as RFLP and CAPS, and was simple, efficient and easy to operate. It was especially suitable for early cytoplasmic genetic composition analysis of somatic hybrids in citrus plants.