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本研究利用SRAP分子标记技术对太行山特有濒危物种太行菊11个自然居群的遗传多样性进行研究。采用10对引物对11个居群的151个样品进行扩增,共得到90个扩增位点,其中多态性位点73个,多态位点百分率(PPL)为81.11%。POPGENE分析显示,太行菊具有较高的遗传多样性(H=0.205 9,I=0.325 6)。Nei’s遗传多样性分析表明,11个自然居群间出现了较高的遗传分化(基因分化系数Gst=0.274 9,基因流Nm=1.318 8)。生境的片段化和基因流障碍可能是导致太行菊居群间遗传分化显著的主要原因。通过对太行菊居群遗传多样性的研究,可为有效保护太行菊提供理论依据。
In this study, genetic diversity of 11 natural populations of Taihang chrysanthemum, a endemic and endangered species in the Taihang Mountains, was studied using SRAP molecular markers. A total of 151 amplified samples were obtained from 151 samples of 11 populations using 10 pairs of primers, of which 73 were polymorphic, with a percentage of polymorphic loci (PPL) of 81.11%. POPGENE analysis showed that Taihang chrysanthemum has a high genetic diversity (H = 0.205 9, I = 0.325 6). Nei’s genetic diversity analysis showed that there was a high level of genetic differentiation among 11 natural populations (Gst = 0.274 9, Nm = 1.318 8). Habitat fragmentation and gene flow disorders may be the main reason for the significant genetic differentiation among populations of Taihang chrysanthemum. By studying the genetic diversity of the population of Taihang chrysanthemum, it can provide a theoretical basis for the effective protection of Taihang chrysanthemum.