三年来,笔者用100ml的医用盐水瓶作容器制母种取得较满意效果。现介绍如下:到医院收集废瓶刷净备用,按常规法配制琼脂培养基,用玻璃漏斗每瓶装20ml加棉塞包扎牛皮纸,立放在手提式灭菌锅内,每层放21瓶,两层之间要加一个铝篦。灭菌后趁热把瓶平冷凝培奍基平面。接种时,菌种块放在瓶底 In the past three years, the author used 100ml medical saline bottles as container-type mother to obtain more satisfactory results. Are introduced as follows: to the hospital to collect waste bottle brush net spare, agar culture medium according to conventional methods, with a glass funnel per bottle 20ml plus tampon wrapped kraft paper, stand in a portable sterilizer, put 21 bottles on each floor, two Between the layers to add an aluminum grate. After the sterilization hot bottle flat coagulation 奍 base plane. Inoculation, bacteria species on the bottom of the bottle