论文部分内容阅读
机体淋巴细胞中起抗肿瘤作用的主要是细胞毒T细胞(CTL),CTL表面的T细胞受体(TCR)可以识别肿瘤细胞表面HLA呈递的抗原肽,攻击和破坏肿瘤细胞。淋巴瘤表面的免疫球蛋白(Ig)作为独特型抗原可以用于免疫治疗,但是CTL如何识别独特型并不清楚。研究发现供CTL识别不同独特型的T细胞表位(一些抗原9肽)主要分别位于Ig重链基因(IgHV)的框架区。人工合成这些T细胞表位,刺激正常人淋巴细胞可以获得大量特异性的抗淋巴瘤的CTL。淋巴瘤按照IgHV可以分为7种基因型。将特异性CTL的TCR基因转染正常T细胞,有可能获得用于免疫治疗的抗不同基因型淋巴瘤的淋巴细胞。
Tumor cell lymphocytes play an antitumor effect mainly cytotoxic T cells (CTL), CTL surface T cell receptor (TCR) can be identified on the surface of tumor cells presenting antigen peptide, attack and destroy tumor cells. Immunoglobulins (Ig) on the surface of lymphoma can be used for immunotherapy as idiotype antigens, but it is unclear how CTL recognizes idiotypes. The study found that CTLs recognize different idiotypes of T cell epitopes (some of the antigen 9 peptides) are mainly located in the Ig heavy chain gene (IgHV) framework. Artificial synthesis of these T-cell epitopes, to stimulate normal human lymphocytes can obtain a large number of specific anti-lymphoma CTL. Lymphoma according to IgHV can be divided into seven genotypes. Transfection of TCR genes of specific CTLs to normal T cells makes it possible to obtain lymphocytes against different genotypes of lymphoma for immunotherapy.