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本文指的电子签象是由电子显微镜得到的电子显微签。这种显微签象给我们直接看到细胞和病毒中的微观结构或者甚至看到分子和晶体中的原子排列的印象。这些用肉眼是直接看不到的。我们实际看到的当然是来自观看屏幕的光发射或照相乳胶的光学密度的一些二维变化。我们发现当工作在接近于极限分辨率时,象不仅与试样有关而且还与仪器的工作条件例如照明、孔阑大小和位置或散焦有很灵敏的关系。
This article refers to the electronic signature electron microscopy electron microscopy. This micro signature gives us the impression of seeing directly the microstructure in cells and viruses or even the atomic arrangement of molecules and crystals. These are invisible to the naked eye. What we actually see is, of course, some two-dimensional change in the optical density of the light emission or photographic latex viewing the screen. We found that when working near extreme resolution, the image is not only sample-dependent but also sensitive to the working conditions of the instrument such as illumination, aperture size and position or defocusing.